Keyword Analysis & Research: replication competent aav

Keyword Analysis

Keyword	CPC	PCC	Volume	Score	Length of keyword
replication competent aav	0.32	0.4	3485	59	25
replication	1.85	0.6	6210	37	11
competent	0.87	0.9	924	32	9
aav	0.68	0.9	8068	94	3

Keyword	CPC	PCC	Volume	Score
replication competent aav	0.78	0.3	2268	21
replication competent aav testing	0.98	0.2	8037	28
replication competent aav assay	1.46	0.9	8522	35
replication competent aav usp	1.03	0.1	7430	50
replication competent aav fda	1.34	0.1	5030	95
replication competent aav fda guidance	1.3	0.4	8594	32
replication competent lentivirus assay	0.42	0.2	2700	69
replication competent lentivirus fda	0.99	0.9	4255	87
replication-competent adenovirus	1.15	0.7	9174	98
replication-competent virus	0.31	0.8	4405	62
replication-competent	0.53	0.9	5934	92
replication-competent lentivirus	0.38	0.7	322	51

Cellular, Tissue, and Gene Therapies Advisory Committee …
fda.gov

https://www.fda.gov/media/151599/download

Webreplicase proteins that are required for AAV replication and packaging of the genome in the capsid. The ITRs are also essential for replication and packaging and form hairpin-like structures.

DA: 52 PA: 98 MOZ Rank: 84

Adeno-Associated Virus (AAV) as a Vector for Gene Therapy
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548848/

WebJul 1, 2017 · Adeno-associated virus (AAV) is a non-enveloped virus that can be engineered to deliver DNA to target cells, and has attracted a significant amount of attention in the field, especially in clinical-stage experimental therapeutic strategies.

DA: 71 PA: 12 MOZ Rank: 35

Identification and elimination of replication-competent adeno
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC191962/

WebAbstract. Adeno-associated virus (AAV) vector preparations are often contaminated with variable amounts of replication-competent AAV (rcAAV), which may influence the behavior of these vectors both in cultured cells and in animals. A packaging plasmid/vector plasmid system containing no significant homology and lacking the wild-type AAV p5 ...

DA: 88 PA: 18 MOZ Rank: 5

Meeting FDA Guidance recommendations for replication-competent …
sciencedirect.com

https://www.sciencedirect.com/science/article/pii/S2329050122001681

WebMar 9, 2023 · The assay needs to be tailored for the parent virus (e.g., MLV, HIV-1) and vector pseudotype (envelope). The FDA has recommended a minimum of five cell passages (approximately 3 weeks) of culture to identify slow-growing viruses. Table 1 provides a list of detection methods used in RCR assays.

DA: 73 PA: 75 MOZ Rank: 50

Identification and elimination of replication-competent adeno
nih.gov

https://pubmed.ncbi.nlm.nih.gov/9261406/

WebAbstract. Adeno-associated virus (AAV) vector preparations are often contaminated with variable amounts of replication-competent AAV (rcAAV), which may influence the behavior of these vectors both in cultured cells and in animals.

DA: 79 PA: 82 MOZ Rank: 31

Replication competent helper functions for recombinant AAV …
nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2638078/

WebReplication competent AAV determination. The titer of wild-type AAV or replication competent AAV was determined by serial dilution of 2 μl cell lysate and infecting 293 cells in the absence or presence of adenovirus infection at MOI of 5. The total cellular DNA was isolated 48 h after infection.

DA: 55 PA: 98 MOZ Rank: 25

Adeno-associated virus as a delivery vector for gene therapy
nature.com

https://www.nature.com/articles/s41392-024-01780-w

WebApr 3, 2024 · AAVone yields higher rAAV production compared to the triple transfection method, with lower batch-to-batch variations and reduced levels of replication-competent AAV (rcAAV).

DA: 6 PA: 85 MOZ Rank: 75

Querying Recombination Junctions of Replication-Competent …
nih.gov

https://pubmed.ncbi.nlm.nih.gov/37376529/

WebMay 24, 2023 · One type of contaminant that remains poorly investigated is replication-competent (rc)AAVs. rcAAVs form through recombination of DNA originating from production materials, yielding intact, replicative, and potentially infectious virus-like virions.

DA: 47 PA: 10 MOZ Rank: 62

Development of an Inducible, Replication-Competent Assay Cell …
nih.gov

https://pubmed.ncbi.nlm.nih.gov/36565023/

WebFeb 1, 2024 · Development of an Inducible, Replication-Competent Assay Cell Line for Titration of Infectious Recombinant Adeno-Associated Virus Vectors. Hum Gene Ther. 2023 Feb;34 (3-4):162-170. doi: 10.1089/hum.2022.001. Authors. Zion Lee 1 , Min Lu 1 , Eesha Irfanullah 1 , Morgan Soukup 1 , Daniel Schmidt 1 2 , Wei-Shou Hu 1. Affiliations.

DA: 74 PA: 70 MOZ Rank: 60

Replication-Competent AAV Testing - Creative Biogene
creative-biogene.com

https://www.creative-biogene.com/Services/Custom-Viral-Service/Replication-Competent-AAV-Testing.html

WebReplication-incompetent vector particles derived from AAV (adeno-associated virus) have been shown to mediate transfer and expression of heterologous genes (transgenes) into a variety of cells in vivo and in vitro. It has tremendous potential in both research and therapeutic applications.

DA: 25 PA: 14 MOZ Rank: 16