Keyword Analysis & Research: n terminal fusion
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Fluorescent Proteins 101: GFP Fusion Proteins …
https://blog.addgene.org/gfp-fusion-proteins-making-the-right-connection
WEBCreating fusions at the N- and C- termini of your protein of interest. The most straightforward fusion protein is made by connecting the N- or C-terminus of the …
DA: 86 PA: 31 MOZ Rank: 71
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Thermodynamic and molecular dynamic insights into …
https://www.nature.com/articles/s41598-024-59355-2
WEBIn fact, it is known that the form of fusion (the selection of N-terminal fusion, C-terminal fusion, and the others) can affect the recognition significantly 1,6,7,8,9.
DA: 79 PA: 16 MOZ Rank: 44
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Investigation Into the use of C- and N-terminal GFP …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447460/
WEBAbstract. Reverse transfection microarrays were described recently as a high throughput method for studying gene function. We have investigated the use of this technology for …
DA: 57 PA: 7 MOZ Rank: 25
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N pro fusion technology to produce proteins with …
https://www.nature.com/articles/nmeth1116
WEBNpro fusion technology to produce proteins with authentic N termini in E. coli | Nature Methods. Article. Published: 18 November 2007. N pro fusion technology to produce …
DA: 9 PA: 80 MOZ Rank: 26
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A comparison between MBP- and NT* as N-terminal …
https://www.sciencedirect.com/science/article/pii/S1046592821001741
WEBOften, fusion proteins are used to increase expression levels, solubility, or both. Here, we compare a recently reported fusion tag, NT*, with Maltose Binding Protein (MBP), a well …
DA: 88 PA: 12 MOZ Rank: 89
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Molecular basis for ubiquitin/Fubi cross-reactivity in …
https://www.nature.com/articles/s41589-023-01388-1
WEBThe immunosuppressive ubiquitin-like protein Fubi is synthesized as an N-terminal fusion to a ribosomal protein (Fubi-S30). Its proteolytic maturation by the nucleolar …
DA: 39 PA: 16 MOZ Rank: 9
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Fusion tags to enhance heterologous protein expression
https://link.springer.com/article/10.1007/s00253-020-10402-8
WEBThe use of a fusion tag is one of the most powerful strategies for obtaining large amounts of heterologous protein in E. coli expression system. Here, recent advances in fusion tags …
DA: 86 PA: 24 MOZ Rank: 23
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N-terminal fusion of the N-terminal domain of bacterial …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942671/
WEBOur method incorporates a novel fusion tag (the N-terminal domain of bacterial enzyme I, EIN) that dramatically increases the solubility of its fusion partner and is promptly …
DA: 74 PA: 41 MOZ Rank: 26
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TSGIT: An N‐ and C‐terminal tandem tag system for …
https://onlinelibrary.wiley.com/doi/full/10.1002/pro.3989
WEBThe system includes two affinity tags, two solubilization tags and two cleavable tags distributed at both termini of the protein of interest. Therefore, the N- and the C-terminal …
DA: 64 PA: 27 MOZ Rank: 72
