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Home - Ivím Health
https://www.ivimhealth.com/
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In vitro genotoxicity testing using the micronucleus assay in cell
https://academic.oup.com/mutage/article/26/1/177/1059280
WEBJan 1, 2011 · The in vitro micronucleus test (IVMNT) has evolved into a robust quantitative assay of chromosome damage by the development of the cytokinesis-block technique that eliminated the confounding effects on MN expression by the cytostatic effects caused by poor culture conditions, treatment effects, cell senescence and variability in mitogen …
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Test No. 487: In Vitro Mammalian Cell Micronucleus Test | en | OECD
https://www.oecd.org/chemicalsafety/test-no-487-in-vitro-mammalian-cell-micronucleus-test-9789264264861-en.htm
WEBJul 4, 2023 · The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division.
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In vitro genotoxicity testing using the micronucleus assay in cell
https://pubmed.ncbi.nlm.nih.gov/21164200/
WEBAbstract. The toxicological relevance of the micronucleus (MN) test is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in …
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Transforming early pharmaceutical assessment of genotoxicity
https://www.nature.com/articles/s41598-021-82115-5
WEBJan 28, 2021 · The in vitro micronucleus (IVM) assay is one of the recommended cytogenetic tests for the assessment of chromosomal damage. The IVM assay assesses DNA damage at the chromosomal level by...
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Development, qualification, validation and application of the Ames …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977537/
WEBApr 12, 2018 · The VITROCELL ® VC10 ® smoke exposure system offers multiple platforms for air liquid interface (ALI), or air agar interface (AAI) in the case of the Ames test exposure to mimic in vivo -like conditions for assessing the toxicological impact of …
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IN VITRO GENOTOXICITY TESTING USING THE ... - MatTek …
https://www.mattek.com/reference-library/in-vitro-genotoxicity-testing-using-the-micronucleus-assay-in-cell-lines-human-lymphocytes-and-3d-human-skin-models/
WEBImplementation of in vitromicronucleus (IVMN) assays in the battery of tests forhazard and risk assessment of potential mutagens/carcinogensis therefore fully justified. Moreover, the final draftof an OECD guideline became recently available for thistest.
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Development and validation of a high-content screening in vitro
https://www.sciencedirect.com/science/article/pii/S138357181100132X
WEBSep 18, 2011 · Scoring of micronuclei in the regulatory in vitro micronucleus (IVMN) assay is a labor-intensive method that is performed manually on microscope slides by trained operators. This manual scoring of micronuclei results in inter-observer and intra-observer variability, with a scorer-specific coefficient of variation between 5.5 and 9.5% [1] .
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Test No. 487: In Vitro Mammalian Cell Micronucleus Test
https://www.oecd-ilibrary.org/environment/test-no-487-in-vitro-mammalian-cell-micronucleus-test_9789264264861-en
WEBThe in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division.
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High-Throughput and High-Content Micronucleus Assay in CHO …
https://link.springer.com/protocol/10.1007/978-1-4939-6346-1_9
WEBAug 13, 2016 · Among these methods, cytokinesis-block micronucleus (CBMN) method is found to be most favored in vitro micronucleus assay (IVMN) because of its ease, speed, reproducibility , and ability to concurrently estimate mitotic delay and lack of uncertainty regarding its effect on baseline genetic damage [ 9 – 12 ].
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