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Immunocytochemistry Protocol Using Anti-GFP Antibodies
https://www.thermofisher.com/us/en/home/references/protocols/proteins-expression-isolation-and-analysis/gfp-protocol/immunocytochemistry-protocol-using-anti-gfp.html
WEBAll six anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis while the rabbit and mouse antibodies are also useful for immunoprecipitation. A summary of the validated applications for all of these antibodies …
DA: 49 PA: 72 MOZ Rank: 31
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Ten Approaches That Improve Immunostaining: A Review …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836139/
WEBJan 26, 2022 · Schematic representation of green fluorescent protein (GFP)-fluorescence emission in a eukaryotic cell: In situ environment, the cellular spread of GFP-emitting fluorescence (left). GFP under chemical fixation with paraformaldehyde (PFA); in some PFA–GFP complexes, a conformational change of GFP is induced, and as consequence GFP … DOI: 10.3390/ijms23031426 Published: 2022/02 Publication: Int J Mol Sci. 2022 Feb; 23(3): 1426.
DOI: 10.3390/ijms23031426
Published: 2022/02
Publication: Int J Mol Sci. 2022 Feb; 23(3): 1426.
DA: 47 PA: 80 MOZ Rank: 90
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A novel protocol to detect green fluorescent protein in
https://www.nature.com/articles/s41598-020-71493-x
WEBSep 4, 2020 · The green fluorescent protein (GFP) is a powerful reporter protein that allows labeling of specific proteins or entire cells. However, as GFP is a small soluble protein, it easily... Author: Valentina Scandella, Rosa Chiara Paolicelli, Marlen Knobloch Publish Year: 2020
Author: Valentina Scandella, Rosa Chiara Paolicelli, Marlen Knobloch
Publish Year: 2020
DA: 18 PA: 70 MOZ Rank: 58
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Optimized protocols for in situ hybridization, …
https://www.sciencedirect.com/science/article/pii/S0065128121000696
WEBJul 1, 2021 · Immunohistochemistry of GFP (brown coloration) was performed on sections of formalin fixed, decalcified and paraffin embedded distal femur from eGFP-positive rats (strain: LEW-Tg(CAG-EGFP)YsRrrc). Different concentrations of proteinase K for epitope retrieval were evaluated. Author: Zelong Dou, Michael Chau, Daniel Muder, Torbjörn Vedung, Ola Nilsson, Ola Nilsson Publish Year: 2021
Author: Zelong Dou, Michael Chau, Daniel Muder, Torbjörn Vedung, Ola Nilsson, Ola Nilsson
Publish Year: 2021
DA: 29 PA: 98 MOZ Rank: 96
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Semiquantitative Methods for GFP …
https://journals.sagepub.com/doi/pdf/10.1177/0192623320964804
WEBImmunohistochemistry (IHC) and RNA in situ hybridization (ISH) for GFP were performed on PFPE serial eye sections and evaluated using semiquantitative methods. On day 43, GFP labeling in both IHC and ISH sections was greatest in the retinal pigment epithelium, compared with other retinal layers in which expression was negative to moderate.
DA: 95 PA: 16 MOZ Rank: 38
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Semiquantitative Methods for GFP Immunohistochemistry …
https://journals.sagepub.com/doi/full/10.1177/0192623320964804
WEBNov 10, 2020 · Simultaneous analysis of IHC and ISH may be needed for comprehensive assessment of gene transduction and protein biodistribution. This study demonstrates approaches for semiquantitative evaluation of IHC and ISH that allow interpretation and reporting of GFP expression in toxicity studies.
DA: 42 PA: 3 MOZ Rank: 63
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Semiquantitative Methods for GFP Immunohistochemistry …
https://pubmed.ncbi.nlm.nih.gov/33167778/
WEBThis study demonstrates approaches for semiquantitative evaluation of IHC and ISH that allow interpretation and reporting of GFP expression in toxicity studies. Keywords: adeno-associated virus vector; gene therapy; green fluorescent protein; immunohistochemistry; in situ hybridization; ocular toxicology; retina.
DA: 78 PA: 7 MOZ Rank: 64
