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EGFP :: Fluorescent Protein Database
https://www.fpbase.org/protein/egfp/
WEBEGFP. a.k.a. enhanced GFP, GFPmut1. EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.
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Excitation and Emission of Green Fluorescent Proteins - Agilent
https://www.agilent.com/cs/library/technicaloverviews/public/excitation-and-emission-green-fluorescent-proteins-5994-3413EN-agilent.pdf
WEBTable 1. Excitation and emission data of GFP variants GFP variant Excitation max (nm) Emission max (nm) Excitation Filter@ Emission Filter@ wtGFP 395 509 400/30# 508/20 360/40 GFPuv 395 509 400/30# 508/20 360/40 Stemmer 395 509 400/30# 508/20 360/20 EGFP 488 509 485/20 530/25 GFP-S65T 489 509 485/20 530/25 Y66H 382 459 360/40 …
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Green Fluorescent Protein (GFP) | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/fluorophores/green-fluorescent-protein.html
WEBGFP can be excited by the 488 nm laser line and is optimally detected at 510 nm. We offer a series of GFP Invitrogen CellLight fusion constructs of signal peptides or cell structure proteins with emGFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments.
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Green fluorescent protein - Wikipedia
https://en.wikipedia.org/wiki/Green_fluorescent_protein
WEBThe GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy ( Renilla reniformis) has a single major excitation peak at 498 nm.
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Green fluorescent protein: A perspective - PMC - National Center …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190146/
WEBJun 28, 2011 · The GFP chromophore can exist as anionic or neutral, which explains the spectroscopic observation of two coincident absorbance and excitation bands [Fig. 3(b)]. The neutral protonated or A form absorbs in the ultraviolet (UV) at about 395 nm, whereas the anionic or B form absorbs at about 475 nm.
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A Guide to Choosing Fluorescent Protein Combinations for Flow
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008483/
WEBBolded histograms (with small black box) indicate detection channel for the fluorescent protein based on its reported max excitation and emission spectra. Sensitivity Index (SI) for each fluorescent protein is indicated (top right of histogram), and was calculated as described in the Materials and Methods .
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GREEN FLUORESCENT PROTEIN (GFP) - University of …
https://www.gonda.ucla.edu/bri_core/gfp.htm
WEBWild type GFP has two excitation peaks, a major one at 395 nm (in the long UV range) and a smaller one at 475 nm (blue) and its emission peak at 509 nm (green). For wild type GFP, it has been found that exciting the protein at 395 nm causes fairly rapid quenching of the fluorescence.
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The Photophysics of Green Fluorescent Protein: Influence of the …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1305246/
WEBDec 21, 2004 · The surprisingly large shift of the emission wavelength after 400 nm excitation is caused by a fast ESPT from the chromophore's tyrosyl group to a proton acceptor, generating a deprotonated R − chromophore form ( Chattoraj et al., 1996; Lossau et al., 1996 ). However, the emission spectra after excitation at 400 nm and 480 nm are …
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Expression and Detection of Green Fluorescent Protein (GFP)
https://link.springer.com/protocol/10.1385/0-89603-481-X:305
WEBUsing a combinatorial mutagenic strategy, a variant of GFP referred to as RSGFP4 was isolated that has a single fluorescence excitation maximum at 490 nm, yet retains an emission maximum similar to wild-type GFP at 505 nm (21,22).
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Bright and stable monomeric green fluorescent protein derived …
https://www.nature.com/articles/s41592-024-02203-y
WEBFeb 26, 2024 · Imaging conditions, GFP, excitation 488 nm, emission 500–550 nm; FusionRed, excitation 561 nm, emission 580–620 nm.
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