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Expansion microscopy: principles and uses in biological research
https://www.nature.com/articles/s41592-018-0219-4
WEBDec 20, 2018 · Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout...
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The Basics of Expansion Microscopy - PMC - National Center for
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880804/
WEBThe protocol described here is a variant of ExM that uses regents and equipment found in a typical biology laboratory and has been optimized for imaging proteins in expanded brain tissues. Keywords: expansion microscopy, fluorescence microscopy, nanoscale imaging, super-resolution imaging, immunohistochemistry. Go to:
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Expansion microscopy - Wikipedia
https://en.wikipedia.org/wiki/Expansion_microscopy
WEBExpansion microscopy (ExM) is a sample preparation tool for biological samples that allows investigators to identify small structures by expanding them using a polymer system. [1] The premise is to introduce a polymer network into cellular or tissue samples, and then physically expand that polymer network using chemical reactions to increase ...
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Expansion Microscopy: Principles and Uses in Biological Research
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373868/
WEBExpansion microscopy (ExM) enables nanoscale imaging of biological specimens on conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent.
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Next-generation expansion microscopy | Nature Methods
https://www.nature.com/articles/s41592-023-01793-3
WEBFeb 10, 2023 · Expansion microscopy (ExM) is a super-resolution imaging method that has gained popularity in recent years. In ExM, a sample is fixed and embedded in a swellable polymer gel. Upon expansion,...
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Expansion microscopy | Science
https://www.science.org/doi/10.1126/science.1260088
WEBJan 30, 2015 · By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM).
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Expansion microscopy: A powerful nanoscale imaging tool for
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600979/
WEBMost recently, Expansion Microscopy (ExM) emerged as an alternative solution to overcome the diffraction limit by physically magnifying biological specimens, including nervous systems. Here, we discuss how ExM works in general and currently available ExM methods.
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A practical guide to optimization in X10 expansion microscopy
https://www.nature.com/articles/s41596-018-0117-3
WEBFeb 18, 2019 · Expansion microscopy is a relatively new approach to super-resolution imaging that uses expandable hydrogels to isotropically increase the physical distance between fluorophores in biological...
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Expansion Microscopy for Beginners: Visualizing Microtubules in
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286065/
WEBJun 4, 2020 · Expansion microscopy (ExM) is an emerging technology that uses common lab equipment and commercially available, inexpensive chemicals to physically magnify biological specimens so that they can be imaged with nanoscale resolution under conventional, diffractionâlimited microscopes.
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Expansion Microscopy: Protocols for Imaging Proteins and RNA ... - PubMed
https://pubmed.ncbi.nlm.nih.gov/30070431/
WEBExpansion microscopy (ExM) is a recently developed technique that enables nanoscale-resolution imaging of preserved cells and tissues on conventional diffraction-limited microscopes via isotropic physical expansion of the specimens before imaging.
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