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ELISA - Wikipedia
https://en.wikipedia.org/wiki/ELISA
The enzyme-linked immunosorbent assay ( ELISA) ( / ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using ...
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ELISA: What It Is, Purpose, Procedure & Results - Cleveland Clinic
https://my.clevelandclinic.org/health/articles/24990-elisa
What is ELISA? ELISA is a common laboratory testing technique that detects and counts certain antibodies, antigens, proteins and hormones in bodily fluid samples. This includes blood, plasma, pee, saliva (spit) and cerebrospinal fluid (CSF). “ELISA” stands for “enzyme-linked immunosorbent assay.” Another name for it is an EIA test.
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Overview of ELISA | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-elisa.html
Introduction. The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies.
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Enzyme-linked immunosorbent assay (ELISA) | British Society for …
https://www.immunology.org/public-information/bitesized-immunology/experimental-techniques/enzyme-linked-immunosorbent-assay
The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum.
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Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf
https://www.ncbi.nlm.nih.gov/books/NBK555922/
Apr 23, 2023 · Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses.[1] In this type of assay, one of the reaction components is nonspecifically adsorbed or covalently bound to the surface of a solid phase, such as a microtiter well, a magnetic particle, or a plastic bead.
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Basic principles and types of ELISA | Abcam
https://www.abcam.com/kits/elisa-principle
ELISA (which stands for enzyme-linked immunosorbent assay) is a technique to detect the presence of antigens in biological samples. An ELISA, like other types of immunoassays, relies on antibodies to detect a target antigen using highly specific antibody-antigen interactions.
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1.17: ELISA - Biology LibreTexts
https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.17%3A_ELISA
Jan 17, 2023 · ELISA (Enzyme-Linked ImmunoSorbent Assay) is an immunologic technique used to detect the presence and concentration of an antigen or antibody in a sample. The power of an ELISA is based on the extreme specificity of the antigen-antibody interaction.
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Enzyme-linked immunosorbent assay (ELISA) - Britannica
https://www.britannica.com/science/enzyme-linked-immunosorbent-assay
Feb 22, 2024 · enzyme-linked immunosorbent assay (ELISA), biochemical procedure in which a signal produced by an enzymatic reaction is used to detect and quantify the amount of a specific substance in a solution. Enzyme-linked immunosorbent assays (ELISAs) typically are used to detect antigens , though they can also be used to detect other substances ...
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Enzyme-Linked Immunosorbent Assay: Types and Applications
https://link.springer.com/protocol/10.1007/978-1-0716-2903-1_1
Feb 17, 2023 · Abstract. Enzyme-linked immunosorbent assay (ELISA) is an immunological assay widely used in basic science research, clinical application studies, and diagnostics. The ELISA technique relies on the interaction between the antigen (i.e., the target protein) versus the primary antibody against the antigen of interest.
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ELISA : Principle, Procedure, Types, Applications and Animation
https://laboratoryinfo.com/elisa/
Mar 7, 2022 · ELISA : Principle, Procedure, Types, Applications and Animation. Enzyme Linked Immunosorbent Assay (ELISA) is a very sensitive immunochemical technique which is used to access the presence of specific protein (antigen or antibody) in …
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