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Frequently Asked Questions

What kind of gel is used for gel electrophoresis?

Gel electrophoresis is used to identify DNA and its fragments. The gel used in this process is usually agarose gel, or alternatively, acrylamide gel. These gels ‘freeze’ the DNA fragments, such that they are studied well under a high resolution.

What is agarose gel electrophoresis?

Agarose gel electrophoresis is one of the most common electrophoresis technique which is relatively simple and straightforward to perform but possesses great resolving power. The agarose gel consists of microscopic pores that act as a molecular sieve which separates molecules based upon the charge, size and shape.

How do you make a gel electrophoresis?

Basic Steps Aragose and the buffer are mixed together and microwaved to create the gel. The gel is then placed in the gel electrophoresis box and buffer solution is poured onto it. In order to prepare the DNA restriction enzymes must be used. DNA is inserted into the holes (as well as the size standard) using a micropipette.

What is the electroforesis del gel used for?

La electroforesis del gel es una técnica ampliamente utilizada en los laboratorios de ciencias de la vida para separar las macromoléculas tales como DNA, ARN, y proteínas. En esta técnica, se separan las moléculas basaron en su talla y carga eléctrica.

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