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High-content CRISPR screening | Nature Reviews Methods Primers
https://www.nature.com/articles/s43586-021-00093-4
WEBFeb 10, 2022 · CRISPR screens are a powerful source of biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications and species. In pooled CRISPR screens,...
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Introduction to CRISPR screening | IDT - Integrated DNA …
https://www.idtdna.com/pages/education/decoded/article/overview-what-is-crispr-screening
WEBNov 6, 2019 · CRISPR screening is used to find a small number of important genes, or genetic sequences, within a massive number of genetic sequences such as the entire genome. CRISPR screening can identify genes that influence many physiological effects, including drug resistance and drug sensitivity.
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A new era in functional genomics screens | Nature Reviews …
https://www.nature.com/articles/s41576-021-00409-w
WEBSep 20, 2021 · We first describe the development of CRISPR-based screening platforms and their use in screens of protein-coding genes using simple read-outs.
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Am I ready for CRISPR? A user's guide to genetic screens
https://www.nature.com/articles/nrg.2017.97
WEBDec 4, 2017 · High-content CRISPR screening. Article 10 February 2022. Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline. Article 10 September 2021. Main. Whereas the first human...
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Genome-scale CRISPRi screening: A powerful tool in engineering
https://www.sciencedirect.com/science/article/pii/S2667370323000218
WEBSep 1, 2023 · Genome-scale CRISPRi screening: A powerful tool in engineering microbiology. Author links open overlay panel. Letian Sun a b. , Ping Zheng a b c. , Jibin Sun a b c. , Volker F. Wendisch d. , Yu Wang a b c. Show more. Add to Mendeley. Share. https://doi.org/10.1016/j.engmic.2023.100089 Get rights and content. Under a Creative …
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Research Techniques Made Simple: CRISPR Genetic Screens
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525197/
WEBCRISPR-based screens demonstrate improved versatility, efficacy, and lower off-target effects compared with approaches such as RNAi ( Ford et al., 2019; Guitart et al., 2016; Schuster et al., 2019 ).
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CRISPR Screening: Molecular Tools for Studying Virus–Host …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618713/
WEBNov 11, 2021 · CRISPR screens are widely used to investigate complex biological processes, including virus–host interactions, in a high-throughput manner. These cutting-edge molecular tools enable whole-genome analysis of host factors, identifying and validating the effects of these factors on viral replication.
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CRISPR Screens: Approaches, Strategies, and Workflow - Synthego
https://www.synthego.com/guide/crispr-methods/crispr-screen
WEBCRISPR Screens: Approaches, Strategies, and Workflow. Introduction to Functional Genomic Screening. Functional genetic screens are a powerful tool for understanding the genetic underpinnings of biological pathways at a systems level.
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Genome-wide CRISPR-Cas9 knockout screens - Wikipedia
https://en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9_knockout_screens
WEBOver recent years, the genome-wide CRISPR screen has emerged as a powerful tool for studying the intricate networks of cellular signaling. Cellular signaling is essential for a number of fundamental biological processes, including cell growth, proliferation, differentiation, and apoptosis.
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Quality control, modeling, and visualization of CRISPR screens …
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0843-6
WEBDec 16, 2015 · While CRISPR screening is a powerful technique, it creates computational challenges that include: (1) how to evaluate the data quality; (2) how to identify gene or pathway hits from the screens and assess their statistical significance; and (3) how to visualize and explore the screening results efficiently.
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