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Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388126/
WEBAug 21, 2021 · CRISPR/Cas-9 gene editing is adopted from acquired immunity in prokaryotes and consists of two elements: guide RNA used to locate (bind) the target DNA to be edited and Cas-9, a protein that essentially cuts …
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CRISPR gene editing - Wikipedia
https://en.wikipedia.org/wiki/CRISPR_gene_editing
WEBCRISPR gene editing (pronounced / ˈkrɪspər / "crisper") is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified. It is based on a simplified version of the bacterial CRISPR - Cas9 antiviral defense system.
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What Is CRISPR Gene Editing and How Does It Work?
https://health.clevelandclinic.org/crispr-gene-editing
WEBApr 24, 2023 · The goal of this gene editing research is to eliminate or correct the mutation that’s causing the health condition, or has the potential to cause one, such as certain cancers. In other research studies, gene editing is being explored so a mutation isn’t passed down to children at birth.
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What is CRISPR/Cas9? - PMC - National Center for …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975809/
WEBApr 8, 2016 · CRISPR/Cas9 is a gene-editing technology which involves two essential components: a guide RNA to match a desired target gene, and Cas9 (CRISPR-associated protein 9)—an endonuclease which causes a double-stranded DNA break, allowing modifications to the genome (see figure 1).
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CRISPR technology: A decade of genome editing is only the …
https://www.science.org/doi/10.1126/science.add8643
WEBCRISPR-Cas9 has been adapted for simultaneous multilocus editing, which is especially useful for editing crop species that can carry multiple copies of the target gene (e.g., hexaploid wheat) ( 71) and for crop domestication, which involves targeting multiple different genes [reviewed in ( 72 )].
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What are genome editing and CRISPR-Cas9? - MedlinePlus
https://medlineplus.gov/genetics/understanding/genomicresearch/genomeediting/
WEBCRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. When infected with viruses, bacteria capture small pieces of the viruses' DNA and insert them into their own DNA in a particular pattern to create segments known as CRISPR arrays.
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The CRISPR tool kit for genome editing and beyond - Nature
https://www.nature.com/articles/s41467-018-04252-2
WEBMay 15, 2018 · CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering,...
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CRISPR/Cas9 therapeutics: progress and prospects
https://www.nature.com/articles/s41392-023-01309-7
WEBJan 16, 2023 · CRISPR/ Cas9 is a highly effective gene-editing tool that is widely used in the scientific community. 6 The CRISPR/Cas9 system evolved naturally in bacteria and archaea as a defense...
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Current applications and future perspective of CRISPR/Cas9 gene editing
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862238/
WEBFeb 21, 2022 · We first explain the basic principles of CRISPR/Cas9 gene editing and introduce several new CRISPR-based gene editing modes. We next detail the rapid progress of CRISPR screening in revealing tumorigenesis, metastasis, …
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Pioneers of revolutionary CRISPR gene editing win chemistry …
https://www.nature.com/articles/d41586-020-02765-9
WEBOct 7, 2020 · But one unique aspect of CRISPR-Cas9 genome editing has been the ease and versatility of the technique, he adds. “CRISPR-Cas made this so much more readily acceptable,” says Collins.
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