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Biotinylation - Wikipedia
https://en.m.wikipedia.org/wiki/Biotinylation
WebIn biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol).
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Biotinylation | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/biotinylation.html
WebBiotinylation is the process of attaching biotin to proteins and other macromolecules. Biotinylation reagents are available for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls and carbohydrates.
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Biotinylation | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/biotinylation.html
WebWhat is biotinylation? Biotinylation is the labeling of biotin to a molecule of interest such as a protein, antibody, peptide, or other biomolecule (Figure 1). Biotinylated proteins typically retain biological activity because the biotin group is relatively small.
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A proximity-dependent biotinylation map of a human cell | Nature
https://www.nature.com/articles/s41586-021-03592-2
WebJun 2, 2021 · Nature - A proximity-dependent biotinylation technique defines the location of more than 4,000 proteins in a human cell, and almost 36,000 proximal interactions between proteins, including those...
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Biotinylation - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/biotinylation
WebMar 3, 2010 · Reversible biotinylation is a valuable tool for tracking internalized PDGF in the endosomal compartments by microscopy. A carefully controlled labeling reaction generates a fully active bt-PDGF that is readily detectable with antibiotin antibodies and secondary antibodies coupled to fluorescent dyes.
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Plasmids 101: Biotinylation - Addgene
https://blog.addgene.org/plasmids-101-biotinylation
WebBiotinylation is the general process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically. Enzymatic biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site of a newly synthesized enzyme.
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Biotinylation - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/neuroscience/biotinylation
WebPerhaps the most common method of DNA biotinylation is through enzymatic incorporation with the use of a biotin-labeled deoxynucleoside triphosphate. First reported by Langer et al. in 1981, the procedure is probably the most popular nonradioactive labeling technique reported for oligonucleotide probes.
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Rapid one-step biotinylation of biological and non-biological surfaces
https://www.nature.com/articles/s41598-018-21186-3
WebFeb 12, 2018 · We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating...
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Biotinylation - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/chemistry/biotinylation
WebThe biotinylation of proteins is the covalent coupling of biotin to an amino acid or carbohydrate moiety of the protein. Biotinylation occurs in a specific group of proteins known as carboxylases. These are enzymes that are important in several metabolic pathways, including amino acid metabolism, fatty acid biosynthesis, and gluconeogenesis.
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Protein Biotinylation: Techniques, Analysis, and Applications
https://www.creative-proteomics.com/resource/protein-biotinylation-techniques-analysis-applications.htm
WebThe process of protein biotinylation typically involves the application of chemical reagents or enzymatic procedures to attach biotin to target proteins. Once proteins bear the biotin label, they become readily detectable, isolatable, and amenable to study in diverse experimental contexts.
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